Neerja Wadhwa

About

I am M.Sc, M.Phil, Ph.D. I had contributed towards improvement of human and livestock health through transgenic technology by generating transgenic mice using genes related to various diseases which may serve as models for drug testing, vaccine development and therapeutics technologies. I am highly skilled in generating transgenic mouse models using techniques like Microinjection, Somatic Cloning and Testicular electroporation. Transgenic animals had been generated for our home institute and for all those other institutes of the country who were interested in generating transgenic animals but do not have costly infrastructure of transgenic facility.
Also worked on isolation, culture and propagation of Spermatogonial Stem cells and their transplantation into infertile recipients for fertility restoration. Well versed with other skillful techniques like Embryonic Stem cell culture, In Vitro Fertilization etc. Presently, working in Confocal Imaging Facility as a Facility Manager.

Employment

• National Institute of Immunology Senior Technical Officer/Facility Manager
  2021 - Present
• National Institute of Immunology Senior Technical Officer, Facility Manager

Education

• Chaudhary Charan Singh Haryana Agricultural University M.Sc.
  1993 - 1996
• University of Delhi PhD
• Chaudhary Devi Lal University MPhil

Projects & Funding

Projects & funding information is unavailable.

Publications (26)

• Evolution and advancements of techniques in generating transgenic animals: From random integration to targeted precision
  Next Research 2026 DOI: 10.1016/j.nexres.2026.101470
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• Nor1 and Mitophagy: An Insight into Sertoli Cell Function Regulating Spermatogenesis Using a Transgenic Rat Model
  International Journal of Molecular Sciences 2025 DOI: 10.3390/ijms26189209
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• Generation of a Transgenic Mouse Model for Investigating Mitochondria in Sperm
  Cells 2025 DOI: 10.3390/cells14040296
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• Generation of a Transgenic Mouse Model for Investigating Mitochondria in Sperm
  2025 DOI: 10.20944/preprints202501.0422.v1
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• Homeobox transcription factor Meis1 is crucial to Sertoli cell mediated regulation of male fertility
  Andrology 2021 DOI: 10.1111/andr.12941
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• Generating Transgenic Animal Models: Recent Technological Advancements
  Essentials of Laboratory Animal Science: Principles and Practices 2021 DOI: 10.1007/978-981-16-0987-9_29
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• An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation
  DNA Research 2017 DOI: 10.1093/dnares/dsw057
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• An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation
  Stem Cell Research & Therapy 2016 DOI: 10.1186/s13287-016-0405-1
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• Novel Sperm and Gonadotropin-releasing Hormone-based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co-immunization of Male and Female Mice
  Molecular Reproduction and Development 2016 DOI: 10.1002/mrd.22743
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• An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation
  2016
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