DC

Dr krishna Mantravadi

Oasis Center for Reproductive Medicine, Monash University, Rajiv Gandhi University of Health Sciences

ORCID iD 0000-0001-7642-6743

About

Skills Summary

Clinical Andrology

➢ Male Infertility Management – Oligo-Astheno-Teratozoospermia, Cryptozoospermia, Azoospermia, Aspermia, Necrozoospermia, Retrograde Ejaculation, Anejaculation, Raised Sperm DNA Fragmentation Index

Andrology


➢ Routine semen analysis
➢ Advanced sperm functional tests
➢ Antisperm antibody testing
➢ Computerised semen analysis (CASA – competent with Hamilton thorne & MICROPTIC SCA systems)
➢ Sperm preparation for Various ART techniques
➢ Sperm preparation after PESA, MESA, TESE and Testicular biopsies
➢ Maturation of sperms from testicular samples
➢ Ionophoric stimulation of sperms with motility problems
➢ Cryopreservation of sperm and Testicular biopsy tissues
➢ Assessment of Testosterone levels by Radio Immuno Assay

Embryology

➢ Gamete Handling: Identification of oocytes from the follicular fluid, oocyte grading
➢ In Vitro Fertilisation (standard Insemination)
➢ In Vitro maturation of oocytes (IVM)
➢ Embryo culture
➢ Embryo assessment & Embryo Grading
➢ ICSI (trained on different equipments like Eppendorff, RI, Narishigi and Leitz and also trained in setting up the micromanipulator and making micromanipulation pipettes)
➢ IMSI
➢ Slow Freezing of gametes and Embryos
➢ Oocyte and Embryo Vitrification
➢ Oocyte and Ovarian tissue cryopreservation
➢ LASER assisted hatching of embryos
➢ Embryo biopsy
➢ PGD/PGS
➢ Orcein staining and fixation of embryos
➢ Media preparation for gamete handling, embryo culture and cryopreservation
➢ Incubator cleaning, maintenance of various lab equipment and sterilisation procedures
➢ Total Quality management in an IVF lab (QC & QA procedures)
➢ Setting up a new IVF lab
➢ Documentation of various procedures and Internal Audit in IVF lab
➢ Trouble shooting
➢ Interpretation of PGD results and counselling support for infertile couples
➢ Genetic counselling in high risk cases
➢ Tissue culture, maintaining cell lines in culture, serial cell passages (basics of stem cell culturing)

My strengths
Honest, intelligent, creative, perfectionist, enjoys a challenge and a self starter

Languages known: English, Hindi, Telugu and Kannada (reading and writing)


AWARDS

• Gold medal for securing high Distinction in all subjects- 2007 – Monash University
• Best outgoing Alumni – 2009 – Monash University and the Australian international student council
• Best poster presentation – Monash Open Day 2008
• Hall of Fame Embryologist of the Year – 2019 – Economic Times India
• ICON of the YEAR Award – ISAR 2019
• BEST Case Presentation – MedEA – Male Infertility Perceptorship – conducted by Merck- 2020
• Best Research Paper – Fertivision 2017
• Best Research paper – ISAR 2019

Employment

  • Oasis Center for Reproductive Medicine Scientific Head & Clinical Embryologist
    2010 - Present

Education

  • Monash University MASTER OF CLINICAL EMBRYOLOGY
    2018 - 2018
  • Rajiv Gandhi University of Health Sciences MBBS
    2000 - 2006

Projects & Funding

Projects & funding information is unavailable.

Publications (41)