DEBAJIT DAS
Assam Agricultural University, CSIR - North East Institute of Science and Technology
0000-0001-7793-861X
About
🔬 About Me:
I am a dedicated Project Scientist at DBT-North East Centre for Agricultural Biotechnology (DBT-NECAB), affiliated with Assam Agricultural University, India. With a strong foundation in molecular biology and a passion for advancing agricultural biotechnology, I have honed my skills through extensive research in plant-insect interactions and CRISPR-mediated genome editing.
🎓 Education & Professional Background:
Ph.D. in Plant Biotechnology from Assam Agricultural University, focusing on understanding the defense response induced by the bruchid beetle in blackgram.
Previous experience as a Senior Project Associate at CSIR-North East Institute of Science and Technology (CSIR-NEIST), Jorhat-785006, Assam, India.
🔍 Research Focus:
In my previous projects, I focused on using the CRISPR/Cpf1 genome editing system to target a gene that is known to affect stress responses in a specific rice cultivar from the North East. Throughout my extensive experience of 5.5 years, which includes 4 years of doctoral research and 1.5 years of project work, I have developed a strong proficiency in a wide range of molecular biology techniques.
đź’ˇ Key Skills & Expertise:
Molecular Biology: Cloning (conventional and Gateway), heterologous protein expression, plant transformation via Agrobacterium, RNAseq data analysis.
PCR Techniques: RT-PCR, qRT-PCR, DNA/RNA/protein extraction, western blotting, ELISA assays.
Microscopy & Spectrophotometry: Light microscopy, spectrophotometric assays.
CRISPR/Cas Genome Editing: sgRNA design, analysis of editing events, molecular and physiological characterization of edited lines.
Bioinformatics: Sanger sequencing data analysis, transcriptome assembly, statistical analysis in R and Graphpad Prism.
Imaging: Live cell imaging using confocal microscopy.
🚀 Passionate & Driven:
I am driven by an insatiable curiosity and a relentless pursuit of knowledge. My enthusiasm for adopting new techniques and enhancing my skill set fuels my ambition to contribute meaningfully to the genetic improvement of crop plants and address pressing agricultural challenges.
📧 Let's Connect:
I am eager to collaborate, share insights, and explore opportunities in the realm of agricultural biotechnology. Feel free to reach out and connect!
Employment
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Assam Agricultural University Project Scientist2023 - Present
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CSIR - North East Institute of Science and Technology Senior Project Associate2021 - Present
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Assam Agricultural University Junior Research Fellow2018 - 2020
Education
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Assam Agricultural University Ph.D.2014 - 2019
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Assam Agricultural University M.Sc.2012 - 2014
Projects & Funding
Projects & funding information is unavailable.
Publications (23)
- Herbivory-induced alterations in cytosolic proteins of pigeon pea (Cajanus cajan) leaves Save
- Uncovering Herbivory Defense Responsive Proteins in Chickpea (Cicer spp.) Through Mass Spectrometry-Based Proteomics Save
- Integrative Omics Approaches to Enhance Crop Tolerance to Metal(loid) Toxicity: Unraveling Molecular Mechanisms and Adaptive Strategies Save
- A novel formulation of rapeseed protein-tea polyphenol conjugate as an edible emulsifier for stabilizing hydrophobic curcumin in aqueous medium Save
- Computational insights and insecticidal activity of black gram defensin proteins against bruchids (Callosobruchus maculatus) Save
- Dynamics of cytosolic and organellar gene transcripts in wild and cultivated genotypes of pigeon pea due to simulated herbivory Save
- High-throughput Metabolomics for Uncovering Plant–Herbivore Interactions Save
- Application of Bioinformatics Tools in Rice Genomics Research Save
- Improved methods for total and chloroplast protein extraction from Cajanus species for two-dimensional gel electrophoresis and mass spectrometry Save
- Screening for brown-spot disease and drought stress response and identification of dual-stress responsive genes in rice cultivars of Northeast India Save