Negi H

About

My research interests encompass molecular biology, protein biochemistry, and structural biology, with a focus on understanding protein degradation mechanisms, particularly within the Proteasome Degradation machinery. I specialize in investigating degradation kinetics, substrate recognition, ubiquitination, and proteolysis, alongside the regulatory role of post-translational modifications in protein turnover. Proficient in molecular cloning, protein purification, immuno-blotting, and multi-angle light scattering, I have recently expanded into cryo-electron microscopy (cryoEM) for unraveling the structural intricacies of the 26S proteasome. Committed to promoting scientific collaboration and open access, I actively engage in conferences, workshops, and collaborative projects to foster knowledge exchange within the scientific community, maintaining an updated ORCID record to reflect my contributions and achievements accurately

Employment

• NCI-NIH Postdoc Fellow
  2021 - Present

Education

• National Centre for Biological Sciences Doctoral
  2015 - 2021

Projects & Funding

Projects & funding information is unavailable.

Publications (11)

• Investigating Enzyme-Substrate Interactions in the Assembly of K48/K63 Heterotypic Ubiquitin Chains
  Biochemistry 2026 DOI: 10.1021/acs.biochem.5c00709
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• Proteasome activity maintains cell-type-specific gene expression
  Cell Reports 2026 DOI: 10.1016/j.celrep.2026.116973
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• Structures of dynamic interactors at native proteasomes by PhIX-MS and cryoelectron microscopy
  2025 DOI: 10.1101/2025.07.31.667872
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• An adaptive peptide-binding site in ubiquitin receptor hRpn13 revealed by structural studies.
  Nature communications 2025 DOI: 10.1038/s41467-025-60843-w
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• CD28 hinge used in chimeric antigen receptor (CAR) T-cells exhibits local structure and conformational exchange amidst global disorder.
  Communications biology 2024 DOI: 10.1038/s42003-024-06770-w
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• Plasticity of the proteasome-targeting signal Fat10 enhances substrate degradation
  eLife 2024 DOI: 10.7554/eLife.91122
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• An engineered cell line with a hRpn1-attached handle to isolate proteasomes
  Journal of Biological Chemistry 2023 DOI: 10.1016/j.jbc.2023.104948
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• A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes
  Biochemical Journal 2020 DOI: 10.1042/bcj20200289
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• The Viral SUMO–Targeted Ubiquitin Ligase ICP0 is Phosphorylated and Activated by Host Kinase Chk2
  Journal of Molecular Biology 2020 DOI: 10.1016/j.jmb.2020.01.021
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• A five-residue motif for the design of domain swapping in proteins
  Nature Communications 2019 DOI: 10.1038/s41467-019-08295-x
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