PRAKASHA GOWDA ALADAHALLI SANNE GOWDA
Also known as: Prakasha
Eurofins BioPharma Product Testing, University of Mysore
0000-0002-0055-7103
About
Professional Summary:
Accomplished in biochemistry, chemistry, protein biochemistry, structural biology, biophysics, molecular biology, enzymology, carbohydrate chemistry and glycobiology.
Professional Experience:
Aug 2017 to present: Principal Scientist, Eurofins Lancaster Laboratories, Inc., 2425
New Holland Pike, Lancaster, PA 17601
July 2011-Aug 2017: Research associate, Department of Biochem & Mol Biology,
Penn State University, College of Medicine, 500 University Dr,
Hershey, PA 17033
Discovery of polymerase-specific dNTP: Identified a dNTP
substrate that is specific for only one DNA polymerase, pol κ.
With this discovery will be able to elucidate the functions of pol
κ in a cell.
Oct 2004-June 2011: Postdoctoral Scholar, Department of Bioch & Mol Biology, Penn
State University, College of Medicine, 500 University drive,
Hershey, PA 17033
Jan 2003-June 2004: Postdoctoral Scholar, Department of Biochem & Mol pharma,
University of Massachusetts Medical School, 364 Plantation
Street, LRB, Worcester, MA 01655
April 2002-Jan 2003: Research Associate, Department of Molecular Biophysics Unit,
Indian Institute of Science, Bangalore, Karnataka 560012, India
Nov 1997-Jan 2002: Ph. D. Student: Department of Chemistry, University of Mysore,
Mysore, Karnataka 570006, India
Patent Filed:
PSU Invention Disclosure No. 2016-4445: N2-Substituted 2’-Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA polymerase k: A. S. Prakasha Gowda and Thomas E. Spratt. Provisional Patent Application Serial No. 62/311,001 [Polymerase Specific Triphosphate Substrate], filed March 21, 2016, and Provisional Patent Application Serial No. 62/361,090, filed July 11, 2016,
Educational Qualification:
2002 Ph.D. Chemistry, University of Mysore, Mysore, India
1997 M. Sc. Biochemistry, University of Mysore, Mysore, India
1991 B. Sc. Biology, University of Mysore, Mysore, India
Publications: 24 (Including Nature, Angewandte Chemie International Edition, JBC, Biochemistry, Chemical research toxicology, European J. of Medicinal chemistry & Cell Death and Disease)
Abstracts/presentations: 30
Research technics expertise:
Biochemical, Biophysical & analytical:
HPLC, Gas chromatography, Densitometer, analytical ultracentrifuge and density gradient centrifugation. Flow cytometry, FACS analysis, Dionex Bio-LC and RQF-3 quenches flow apparatus (Kin Tek). UV Spectrophotometry, Circular Dichroism, Isothermal Titration Calorimeter (ITC). BiAcoreTM[Surface plasmon resonance (SPR) Technology]. FlexStation 3 Multi-Mode (Molecular Devices) and Softmax Pro 5 microplate readers.
Chip Sequence:
• Next generation gene sequencing experiments. DNA sequencing technology
such as MiSeq, HiSeq-2000 and 2500.
• MiSeq/HiSeq result analysis by using Galaxy, Integrative Genomic Viewer (IGV)
and BLAST.
Click Chemistry:
• “Click Reactions” using biotin-conjugated azides and azides
• In vitro and in vivo (live cells) biomolecules labeling by “Click Chemistry”.
Cell Culture:
• cancer, normal, stem cells, Insect cells (SF9 cells), baculovirus, human blood
lymphocytes, mouse embryo fibroblast cells & malarial parasite .
Protein chemistry purification/characterization:
• Protein purification (bacterial, mammalian, insect cells) & characterization. SDS-
PAGE and Immunoblotting. qubit, Nano drop measurements &
plate reader assays.
• Isolation of proteoglycans from human placenta and animal tissue.
• Photo-affinity probe preparation. Radiolabeling of proteins, oligosaccharides & DNA using I125, S35, P32 & H3.
Enzymology:
• Enzyme assays development, Kd, kcat/Km measurements and inhibition studies.
• DNA repair proteins and DNA polymerases, enzyme–DNA binding constants & kinetics of reactions.
• Enzyme pre-steady state, steady state kinetics & single turnover experiments. Fluorescence polarization/anisotropy.
Molecular Biology and Immunology
• Transfection, DNA isolation, western blot, PCR and RT-PCR. Immunofluorescence, immunoprecipitation, ELISA & immunohistochemistry.
DNA ligations, enzyme digestions & DNA shear by Covaris.
Proteomics:
• Mass spectrometry MALDI, LC-MS & MALDI-TOF.
Peptide synthesis and characterization :
• Peptide synthesis, kinetics of peptide analogs to probe the antimicrobial activity.
Mice work: in vivo and in vitro studies.
Employment
-
Eurofins BioPharma Product Testing Principal Scientist2017 - Present
Education
-
University of Mysore Ph.D? - 2002
Projects & Funding
Projects & funding information is unavailable.
Publications (24)
- Effect of Excipients on Recombinant Interleukin-2 Stability in Aqueous Buffers Save
- A simple RP-HPLC method for the stability-indicating determination of N-acetyl-L-cysteine and N,N’-diacetyl-L-cystine in cell culture media Save
- Active Site Interactions Impact Phosphoryl Transfer during Replication of Damaged and Undamaged DNA by Escherichia coli DNA Polymerase I Save
- Design, synthesis, and identification of a novel napthalamide-isoselenocyanate compound NISC-6 as a dual Topoisomerase-IIα and Akt pathway inhibitor, and evaluation of its anti-melanoma activity Save
- Honokiol Inhibits DNA Polymerases β and λ and Increases Bleomycin Sensitivity of Human Cancer Cells Save
- N2-Substituted 2′-Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase κ Save
- DNA Polymerase ν Rapidly Bypasses O6-Methyl-dG but Not O6-[4-(3-Pyridyl)-4-oxobutyl-dG and O2-Alkyl-dTs Save
- DNA Polymerases η and ζ Combine to Bypass O2-[4-(3-Pyridyl)-4-oxobutyl]thymine, a DNA Adduct Formed from Tobacco Carcinogens Save
- Human DNA Polymerase ν Catalyzes Correct and Incorrect DNA Synthesis with High Catalytic Efficiency Save
- Interaction of Sirt3 with OGG1 contributes to repair of mitochondrial DNA and protects from apoptotic cell death under oxidative stress Save