RV

Rajesh Vinnakota

Centre for Cellular and Molecular Biology, Northwestern University Feinberg School of Medicine, Maastricht University

ORCID iD 0000-0002-2354-5105
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About

I am an experienced drug screening (electrophysiology) professional with over 15 years of experience, including 5 years of CRO-based research and 10 years of academic research. I bring efficient technical expertise and a strong record of achievements. My proven experience includes lab setup, team management, lab operations, cross-functional collaborations, problem-solving, troubleshooting, project planning, and taking on end-to-end responsibilities. I have worked with both automated and manual patch-clamp setups to understand the effects of various pharmaceutical molecules on ion channels.

RESEARCH SKILLS
Expertise in neuroscience research, with a focus on inotropic glutamate receptor structural and functional studies.
Proficient in electrophysiological techniques such as patch clamp recording, voltage, and current clamp recordings
Electrophysiology (In vitro and In vivo Patch-Clamp technique) overexpressed HEK cells (Whole-cell, out-side out patch-clamp) electrophysiology – Brain Slice electrophysiology
Knowledge of various experimental designs and protocols for studying ion channels, synaptic transmission, and neuronal excitability
Familiarity with surgical techniques for in vivo and in vitro electrophysiological recordings
Strong troubleshooting and problem-solving skills, with a track record of successful data acquisition and analysis
Conducted patch-clamp experiments to record and analyze ion channel activity in primary neurons (ex. Dorsal Root Ganglia)
Utilized various molecular biology techniques such as DNA isolation, PCR, and cloning to create constructs for transfection and gene expression studies

EXPERTISE
Experience in performing and analyzing single-channel recordings and pulled patch experiments for ion channels using the patch clamp technique
High success rate with Whole-cell configurations
Skilled in fast ligand application techniques for pulled patch configurations, including outside-out and inside-out patches.
Making suitable ligand application microflow pipettes (double and tribble barrels) for accurate fast-solution exchange
Detection and minimizing the electrical noise associated with grounding
Developing suitable protocols for various critical experimental needs to understand the physiologically relevant properties of ion channels, including dose response, desensitization, deactivation, and recovery from desensitization

Employment

  • Centre for Cellular and Molecular Biology Postdoctoral Scientist
    2023 - Present
  • Northwestern University Feinberg School of Medicine Postdoctoral researcher
    2021 - Present
  • Maastricht University EMBO-STF
    2019 - 2019
  • National Centre for Cell Science Postdoctoral Fellow
    2016 - 2021
  • AU-KBC Research Centre, Madras Institute of Technology, Anna University Senior Research Fellow
    2012 - 2015
  • Acharya N. G. Ranga Agricultural University Senior Research Fellow
    2010 - 2011

Education

Education history is unavailable.

Projects & Funding

Projects & funding information is unavailable.

Publications (16)