Rakesh Kumar Singh

About

Assistant Professor, Department of Biotechnology, Vivek University, Bijnor,uttar Pradesh, India

Research Focus: "Isolation and Characterization of Novel Lytic Bacteriophages Against Enterococcus faecium and Its Clinical Isolates"
Research on phage therapy and its potential applications in treating infections such as urinary tract infections, bacteremia, and biofilm disruption or inhibition using bacteriophages. Collaborating with the Institute of Medical Sciences, Banaras Hindu University. I have Submitted two Enterococcus faecium phages to the NCBI database:

RG1 Phage (Accession ID: PQ586903, December 10, 2024)
SSBHU Phage (Accession ID: PQ778307, February 4, 2025).

My Doctoral research focused on understanding how housekeeping (SigA) and alternative sigma factors (SigB) regulate transcription and bacterial adaptation in adverse environmental condition in Mycobacterium smegmatis. I performed in silico characterization of sigma factor classes across Mycobacteria where I found interesting outcome, which includes motif, domain and conserver amino acid residue followed by cloning, expression, and purification of SigA and SigB. Using biophysical analysis, I demonstrated distinct stability patterns due to different secondary structural composition of peptides alpha helical and beta pelted sheet. Finally, through affinity purification–HRMS proteomics, I mapped the interactomes of SigA and SigB during log and stationary phases, identifying key regulators that involve in the regulation of metabolic pathways they. This work provides important insights into transcriptional regulation, stress response, and bacterial survival mechanisms.

Employment

• Patanjali Research Foundation Research Associate
  2024 - 2025
• Banaras Hindu University Postdoc fellow
  2023 - 2024
• Institute for Stem Cell Biology and Regenerative Medicine Research Associate
  2023 - 2023

Education

• Banaras Hindu University Doctor of Philosophy in Plant Biotechnology
  2016 - 2023

Projects & Funding

Projects & funding information is unavailable.

Publications (9)

• Isolation, functional characterization and antibiofilm properties of a lytic Enterococcus phage RG1 against multidrug resistant E. faecium
  Scientific Reports 2025 DOI: 10.1038/S41598-025-21701-3
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• A comparative analysis of mycobacterial ribonucleases: Towards a therapeutic novel drug target
  Infection, Genetics and Evolution 2024 DOI: 10.1016/J.MEEGID.2024.105645
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• Heterologous Expression, Purification and Structural Characterization of Ribonuclease E from Mycobacterium smegmatis
  Iranian Journal of Science 2023 DOI: 10.1007/s40995-023-01467-x
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• Isolation and characterization of a novel mycobacteriophage Kashi-VT1 infecting Mycobacterium species
  Frontiers in Cellular and Infection Microbiology 2023 DOI: 10.3389/fcimb.2023.1173894
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• Expression, Purification, and In Silico Characterization of Mycobacterium smegmatis Alternative Sigma Factor SigB
  Disease Markers 2022 DOI: 10.1155/2022/7475704
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• Effects of Salt Stress on Nutrient Cycle and Uptake of Crop Plants. Physiology of Salt Stress in Plants
  Perception, Signalling, Omics and Tolerance Mechanism 2021
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• A Survey of Recent Nanotechnology-Related Patents for Treatment of Tuberculosis.
  In Intellectual Property Issues in Nanotechnology 2020
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• BACTEROPHAGE ENCODED ENDOLYSINS AS POTENTIAL ANTIBACTERIALS
  Journal of Scientific Research 2019
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• Isolation and functional characterization of a lytic phage SSBHU against Enterococcus faecium and its potential application in phage therapy
  Biologia
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