DG

DEBABRATA GHOSH DASTIDAR

Guru Nanak Institute of Pharmaceutical Science & Technology, Guru Nanak Institute of Pharmaceutical Science and Technology, Yoga Vidya Gurukul, University of Calcutta, Jadavpur University

ORCID iD 0000-0002-4762-7295
Follow

About

I have completed my Graduation (B.Pharm., 2007) and post-graduation (M.Pharm., 2009) in Pharmaceutical Science and Technology from Jadavpur University (http://www.jaduniv.edu.in/), Kolkata, WB, India. In M.Pharm. I worked on the synthesis and characterization of solid lipid nanoparticles as drug delivery vehicles. After completion of M.Pharm. I joined as Assistant Professor at Guru Nanak Institute of Pharmaceutical Science and Technology (www.gnipst.ac.in), WB, India. Due to my immense interest in material chemistry and biological science, I also joined the Ph.D program in the Department of Biotechnology, Calcutta University (https://www.caluniv.ac.in/), WB, India in 2011. My thesis was ‘Production and characterization of paclitaxel loaded solid lipid nanoparticle to increase the potency and therapeutic efficacy. My findings have been published in Nanomedicine (https://dx.doi.org/10.2217/nnm-2018-0419) and patented in India (Application No.:
201831016759 A). I have been awarded Ph.D on 4th January 2021.

In my doctoral thesis, I explored the chemistry behind the synthesis of core-shell nanoparticles of cetyl alcohol to encapsulate lipophilic drugs like paclitaxel as core material. The process parameters were studied with statistically designed experiments using Design Expert softwares. The optimized nanoparticles had ~78 nm diameter (measured with DLS, AFM, EFM) with ~99% encapsulation efficiency, as estimated by HPLC. The core-shell structure was confirmed by TEM. Interestingly, this product was not only very effective against ALDH+ cancer cells but also against the paclitaxel-resistant A549 cells. When tested in tumor-bearing Balb/c mice, the effect of oral administration of this nanoformulation was comparable to that of the intravenously administered nab-paclitaxel (FDA-approved nanoformulation). The nanoformulation had less systemic toxicity, and a better pharmacokinetic profile in SD rats in comparison to Taxol® and nab-paclitaxel. Actually, the nanoparticles are completely absorbed via lymphatic channels, get accumulated in tumour tissue by passive targeting, and are rapidly uptaken by cancer cells by endocytosis. It is followed by rapid degradation of lipid shell (cetyl alcohol) by the alcohol dehydrogenase (ADH) and Alcohol dehydrogenase (ALDH) enzymes that are
overexpressed in cancer cells. This triggers the rapid release of paclitaxel within the cytoplasm leading to apoptosis and cell death.

During this journey, I have worked on multiple projects where the molecular mechanisms behind the anticancer activity of different molecules like NMK-BH2, apocynin, NMK-T-057, Theaflavin, epigallocatechin-3-gallate, and apigenin were tested. The works have been published in reputed journals like Phytomedicine, Biochimie, Journal of Biological Chemistry, Biochimica et Biophysica Acta (BBA) – General Subjects, Biophysica Acta(BBA) - Molecular Cell Research, and Journal of Cellular Biochemistry. In these projects, my contribution was in designing, performing, and data analysis of cell biological experiments and experimental animal models for breast cancer. I had also done the Insilico prediction of the binding site of ligands to the target proteins by computational chemistry tools. Recently, our team have published an article entitled ‘Computational prediction of the molecular mechanism of statin group of drugs against SARS-CoV-2 pathogenesis’ in Scientific Reports (https://doi.org/10.1038/s41598-022-09845-y). I have a major contribution to this work by performing the virtual screening and molecular dynamics studies.

I have also contributed to many other projects of my collaborators where the antimicrobial and antibiofilm activities of many natural compounds like morin, tryptophan, caffeine, thymoquinone etc. had been tested. Our findings have been published in reputed journals like Archives of Microbiology, 3 Biotech, and Folia Microbiologica.

I have guided more than Fifty M.Pharm. students in the completion of their project work on different areas like formulation and optimization of sustained-release solid dosage forms, self-emulsifying drug delivery systems, transdermal patch; nano-formulations like lipid nanoparticles, polymeric nanoparticles, lipid-polymer hybrid nanoparticles, liposome; nanomaterials like hydrophilic carbon dot, hydrophobic carbon dot, metal nanoparticles, metal-polymer conjugates; microparticles like polymeric microsphere, and polymeric porous microsphere, porous metal oxide nanomaterials. Few notable publications are: https://doi.org/10.1016/j.colsurfa.2020.125781 (carbon dot for Zn ion sensing), https://doi.org/10.1088/2053-1591/ab637f (silver nanoparticle conjugated chitosan microsphere), https://doi.org/10.1088/2053-1591/ab637f (porous chitosan microsphere with surface conjugated carbon dot and silver nanoparticle).

Employment

  • Guru Nanak Institute of Pharmaceutical Science & Technology Associate Professor
    2021 - Present
  • Guru Nanak Institute of Pharmaceutical Science and Technology Assistant Professor
    2009 - 2021

Education

  • Yoga Vidya Gurukul Diploma in Yoga Sastra (Yoga Shikshak)
    2021 - 2022
  • University of Calcutta Ph.D
    2011 - 2021
  • Jadavpur University M.Pharm
    2007 - 2009
  • Jadavpur University Bachelor in Pharmacy (B.Pharm.)
    2003 - 2007

Projects & Funding

Projects & funding information is unavailable.

Publications (38)