Manjari Shukla

About

I am working in the Structural biology lab group and studying the molecular mechanism of bacterial pathogenesis and drug resistance.
My core competencies are:
• In-silico Pharmacophore based drug designing and Receptor guided drug designing
• Molecular Dynamics Simulation studies
• Experience in nanoparticle synthesis and characterization using FTIR, DLS, SEM and TEM
• Molecular Biology Techniques: Cloning, Expression and Purification of proteins (Ni-NTA, GFC, IEC)
• Protein Characterization and Assay Development: SDS PAGE, UV-Vis spectroscopy, Enzyme kinetics, Circular dichroism, Fluorescence spectroscopy, ITC
• Microbiology techniques: Cell culture, Microtiter plate assay, Biofilm forming studies, Phytochemical extraction and characterization
• Protein crystallization (Hanging drop and Sitting Drop vapour diffusion), optimization of crystallization conditions
• X-Ray diffraction data collection and structure solution of Single protein crystals
• Proficient in various basic lab techniques
• Currently learning Cryo-EM

Employment

• Indian Institute of Technology Delhi Project Research Scientist II
  2026 - Present

Education

• Indian Institute of Technology Jodhpur PhD
  2020 - 2025
• Institute of Engineering and Technology Lucknow M.Tech
  2017 - 2019

Projects & Funding

Projects & funding information is unavailable.

Publications (14)

• Shaping the active site from a distance: Unravelling the regulatory role of the conserved N-terminal β-flap of thiol peroxidases through structure-function characterization of the Staphylococcal ortholog
  2025 DOI: 10.64898/2025.12.11.693838
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• Deciphering the Structure and Mechanism of SaGpx: A Non-Canonical Glutathione Peroxidase from Staphylococcus aureus
  2025 DOI: 10.1101/2025.11.24.690144
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• BSA-ICG-Cu(ii) complex as an NIR-responsive multifunctional platform for wound healing: deciphering therapeutic action in vitro
  RSC Advances 2025 DOI: 10.1039/D5RA00155B
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• One-Pot Green Synthesis and Biological Evaluation of Dimedone-Coupled 2,3-Dihydrofuran Derivatives to Divulge Their Inhibition Potential against Staphylococcal Thioredoxin Reductase Enzyme
  ACS Omega 2024 DOI: 10.1021/acsomega.4c04325
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• Molecular Mechanisms Driving and Regulating the AAA+ ATPase VCP/p97, an Important Therapeutic Target for Treating Cancer, Neurological and Infectious Diseases.
  Biomolecules 2023 DOI: 10.3390/biom13050737
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• Polyphenol MHQP as an allosteric inhibitor of Kinesin-5: Cease the molecular catwalk of “Drunken Sailor”
  Indian Journal of Biochemistry and Biophysics 2023 DOI: 10.56042/ijbb.v60i9.4056
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• The rapid construction and biological evaluation of densely substituted pyrrolo[1,2-a]indoles via a BF3·OEt2-assisted cascade approach
  Organic & Biomolecular Chemistry 2023 DOI: 10.1039/D3OB01457F
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• Anti-SARS-CoV-2 potential of Cissampelos pareira L. identified by connectivity map-based analysis and in vitro studies
  BMC Complementary Medicine and Therapies 2022 DOI: 10.1186/s12906-022-03584-3
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• Effective Synthesis and Biological Evaluation of Functionalized 2,3-Dihydrofuro[3,2-c]coumarins via an Imidazole-Catalyzed Green Multicomponent Approach
  ACS Omega 2022 DOI: 10.1021/acsomega.2c05361
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• Quorum Quenching-Guided Inhibition of Mixed Bacterial Biofilms and Virulence Properties by Protein Derived From Leaves of Carissa carandas
  Frontiers in Cellular and Infection Microbiology 2022 DOI: 10.3389/fcimb.2022.836819
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