PS

Prakhar Srivastava

Pusan National University, Vellore Institute of Technology University, SRM Research Institute Kattankulathur, Jaypee University of Information Technology

Research areas: Medicine · Microbiology · Bacterial biofilms and quorum sensing · Antibiotic Resistance in Bacteria · Antimicrobial Resistance in Staphylococcus

ORCID iD 0000-0002-6784-4289
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About

Microbiology is one of the quickly turning fields holding applications from the diagnosing, industrial enzymes, and intervention of medical conditions. Due to the possible impact on human life, the importance of microbiology is presently really high in both the populace and scientific docket. The journey of my entire microbiology research started during my undergraduate time.

The experience and skill gained during undergraduate and postgraduate courses in the field of beneficial microbes (Lactobacillus sp) and microbial physiology further made me inclined towards working with pathogenic microbes, as pathogenic microbes share varying degrees of potent virulence factors and have entirely different genetic makeup as compared to other free-living microbes, and the area of antimicrobial resistance is a challenging because of the intrinsic robust nature of pathogens.

During my Ph.D. tenure, I gained expertise in clinical microbiology (Drug-resistant culture handling), antibiotics synergy testing (checkerboard analysis and time-kill analysis), molecular biology (Bacterial cloning, protein overexpression, and purification) WGS (Nanopore sequencing and sequence annotation) and RT-PCR analysis. My current research experience has enabled me to attain in-depth knowledge about quorum sensing autoinducer (C4-HSL and 3-oxo-C12-HSL) purification and quantification using TLC and HPLC, biofilm architecture of pseudomonas aeruginosa (pel polysaccharide and psl), its virulence determinants (rhamnolipid, elastase, protease, pyocyanin, and EPS), various metabolic and drug-resistant pathways which includes-efflux pump, porins, and motility genes, which have a role in chronic infection of drug-resistant pseudomonas isolates.

My future plan is to work on infectious microbiology focusing on biofilm chronicity, antimicrobial resistance mechanism related to quorum sensing and biofilm matrix, and use various biofilm control strategies, novel treatment approach,es and drug formulation (novel peptide synthesis), and various therapeutic markers for antimicrobial treatments. The working experience with infectious pathogens like- pseudomonas aeruginosa has given me knowledge regarding intracellular mechanisms related to biofilm formation and the drug resistance mechanism of infectious pathogens.

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Education

Projects & Funding

  • CSIR-SRF Council of Scientific and Industrial Research

Publications (7)