Niccolò Paoletti
Università degli Studi "G. d'Annunzio" Chieti-Pescara, University of New South Wales, University of Florence
0000-0002-6874-9419
About
Scholarship holder at the Pharmacy Department, University of Chieti, Italy. Degree in Pharmacy and PhD cum laude in Medicinal Chemistry at the University of Florence, Italy.
I developed multi-disciplinary expertise in:
1. Rational design and synthesis of small molecules of pharmaceutical interest. My main research topics are the metalloenzymes carbonic anhydrases (CAs) and the discovery of modulators for biomedical purposes. I achieved excellent knowledge of organic chemistry used for the synthesis of a multitude of CA inhibitory scaffolds and molecular hybrids.
2. In silico structure-based drug design and study of the action mechanisms of small molecules toward their biological targets (proteins and nucleic acids). I have documented experience in the use of computational chemistry and molecular graphics for the computationally driven design of bioactive molecules and for deciphering their mechanism of action, adopting a plethora of molecular modeling techniques.
3. In vitro studies for the biological evaluation of the inhibitors/activators activity on CA isoforms, using the Stopped-Flow kinetic technique. I also performed the activity characterization of new CAs and spectrophotometry studies to detect CA-catalyzed reactions.
4. Structural X-ray resolution of non-canonical structures of nucleic acids. My PhD studies are focused on the resolution of G-quadruplex (G4) and Holiday Junction (HJ) structures in complex with small molecules that act as stabilizers.
5. Mass spectrometry analysis of ligand-target complexes. During my period at the University of New South Wales in Sydney, I learned MS techniques for the determination of dissociation constants of G4 in complexes with stabilizer compounds.
Employment
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Università degli Studi "G. d'Annunzio" Chieti-Pescara Scholarship holder2024 - Present
Education
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University of New South Wales Visiting PhD student2023 - 2024
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University of Florence PhD cum laude2021 - 2024
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University of Florence Master degree2016 - 2021
Projects & Funding
Projects & funding information is unavailable.
Publications (21)
- Targeting Metabolic and pH Regulatory Pathways in Cancer via Dual Inhibition of Nicotinamide Phosphoribosyltransferase and Carbonic Anhydrases IX and XII Save
- Production, crystallographic studies, and functional profiling of γ-carbonic anhydrase from the probiotic Limosilactobacillus reuteri: In vitro and cell-based insights Save
- Synthesis and Biological Evaluation of Indole‐Benzene Sulfonamides as Carbonic Anhydrase II, IX, and XII Inhibitors Save
- Hybrid and Chimeric Heterocycles for the Inhibition of Carbonic Anhydrases Save
- Structural insights into Arylidenehydrazinyl Benzenesulfonamides as potent mycobacterial carbonic anhydrase inhibitors Save
- Novel 3-Sulfonamide Dual-Tail Pyrrol-2-one Bridged Molecules as Potent Human Carbonic Anhydrase Isoform Inhibitors: Design, Synthesis, Molecular Modeling Investigation, and Anticancer Activity in MeWo, SK-BR-3, and MG-63 Cell Lines Save
- Exploring aliphatic sulfonamides as multiclass inhibitors of the carbonic anhydrases from the pathogen bacterium Vibrio cholerae Save
- Exploration of New Pyrazole–Hydrazone–Benzenesulfonamide Conjugates as Potent Mycobacterial Carbonic Anhydrase Inhibitors: Design, Synthesis, and Biological Evaluation Save
- HIV protease and its inhibition Save
- Sordaria macrospora carbonic anhydrases Save