AP

Aniruddha Panda

Tata Institute of Fundamental Research, Yale University, National Centre for Biological Sciences

ORCID iD 0000-0002-9447-9547
Follow

About

Aniruddha Panda, PhD
Male. D.O.B – 27th March 1985

RESEARCH EXPERIENCE
Yale University, U.S.A
Associate Research Scientist- June 2024 - May 2025
Post-Doctoral Associate- June 2019 – May 2024
Prof. James E. Rothman’s Lab
Prof. Kallol Gupta’s Lab (https://www.theguptalab.com/)


National Centre for Biological Sciences, TIFR, India
Prof. Raghu Padinjat’s Lab (http://flyfat.ncbs.res.in/)
Bridging Post-Doctoral Fellow- December, 2018 – March, 2019
• Signaling role of Phosphatidic acid
Senior Research Fellow - 2013 – 2018
● Biochemical analysis of Phospholipase D in lipid homeostasis in Drosophila phototransduction
My research has focused on revealing fundamental mechanism underlying the role of Phospholipase D (PLD) in regulating lipid levels. We find that photoreceptors have a light-dependent PLD activity that is required to maintain phosphatidic acid levels during G-protein coupled receptor (GPCR) activation and signaling. PLD works in coordination with retromer function and Arf1 activity to regulate apical membrane size.
Junior Research Fellow - 2011 - 2013
● Standardization of mass spectrometry based Lipidomics to identify and quantify different glycerophospholipid molecules involved in Drosophila phototransduction.

The project involved optimization of highly efficient extraction of different glycerophospholipids from cells and intact tissues of Drosophila and total lipid phosphate assay for efficiency checking and data normalization. Later, my research was focused on development of direct infusion mass spectrometry using high resolution Orbitrap technology to identify and quantify individual molecules of glycerophospholipids. During this period, I have successfully characterized lipid regulation by different enzymes involved in Drosophila phototransduction. This method identifies lipids based on class specific chemical formula generated from their monoisotopic masses, so the exact composition of the fatty acyl chains could not be determined. To resolve this issue, I have developed a novel mass spectrometry-based method which couples LC-separation, multiple reaction monitoring and enhanced product ion scan to identify and quantify individual molecular species of glycerophospholipids with the exact composition of fatty acyl chains in sn-1 and sn-2 carbon (regioisomers).

National Centre for Biological Sciences, TIFR, India
Dr. Mrinalini Puranik’s Lab (https://www.ncbs.res.in/former-faculty/puranik)
Junior Research Fellow - 2010 - 2011
● Structural characterization of Tau protein using deep UV resonance Raman spectroscopy along the pathway to fibrillation

Central Glass and Ceramic Research Institute, CSIR, India
Project Assistant (Level II) - 2009 - 2010
● Development of sol-gel based low-e Indium Tin Oxide coatings on glass for different applications

SKILLS

● Native Mass Spectrometry
● Direct Infusion High Resolution Mass Spectrometry (Direct Infusion HRMS)
● Liquid Chromatography-Mass Spectrometry (LC-MS)
● Liquid Chromatography-Multiple Reaction Monitoring (LC-MRM)
● Liquid Chromatography-Multiple Reaction Monitoring-Enhanced Product Ion scan Mass Spectrometry (LC-MRM-EPI-MS)
● Lipidomics
● Multivariate data analysis
● Protein expression & purification
● Basic biochemical analyses
● MS Excel
● Origin
● Adobe Illustrator


EDUCATION
PhD
National Centre for Biological Sciences (NCBS) & Manipal Academy of Higher Education, India.

2013 - 2019

Thesis Title: Biochemical Analysis of Phospholipase D in lipid homeostasis in Drosophila.


M.Sc. (Chemistry)
National Institute of Technology, Rourkela, India

2007 - 2009

Thesis Title: Preparation & Characterization of Mesoporous CeO2-ZrO2 Nanopowders using Dodecylamine & Sodium Dodecyl Sulfate as a Surfactant.

The work involved synthesis and analysis of the structure of Zr4+ doped mesoporous CeO2 nanopowders using different surfactants under different condition. The XRD data showed that mesoporous CeO2-ZrO2 nanopowders were sunthesized in cubic fluorite structure. FTIR results showed that the material was surfactant free and SEM analysis confirmed phase transformation.
http://ethesis.nitrkl.ac.in/154/1/aniruddha_e_thesis.pdf

INSTRUMENTS SKILLS

● Thermo Q Exactive UHMR
● Thermo Orbitrap XL
● Thermo Q Exactive
● Waters Synapt G2 HDMS
● Hybrid triple quadrupole ion-trap mass spectrometer (6500 QTRAP, ABSciex)
● Waters nanoACQUITY UPLC
● Waters Acquity UPLC I Class
● Advion TriVersa NanoMate

Employment

  • Tata Institute of Fundamental Research Reader - F
    2025 - Present
  • Yale University Associate Research Scientist
    2024 - 2025
  • Yale University Post-Doctoral Associate
    2019 - 2024

Education

  • National Centre for Biological Sciences PhD
    2010 - 2019

Projects & Funding

Projects & funding information is unavailable.

Publications (15)