Sheetal Paliwal

About

I am a PhD researcher in chromosome biology, investigating the molecular mechanisms that regulate cohesin dynamics and chromosome organization during meiosis. My research focuses on understanding how distinct dynamic and chromatin-stable populations of cohesin contribute to chromosome function, with particular emphasis on the meiotic cohesin complex and its functional regulation.

My work combines yeast genetics, molecular and cell biology, chromatin-based approaches, and quantitative live-cell fluorescence microscopy. I have developed expertise in fluorescence recovery after photobleaching (FRAP) and single-molecule tracking to quantitatively characterize protein mobility, chromatin association, diffusion behaviour, and residence times in living cells. I also use targeted protein-depletion approaches, cytological analyses, and chromosome segregation assays to connect molecular dynamics with chromosome function.

My broader research interests include cohesin and SMC complexes, chromosome organization, chromatin dynamics, 3D genome architecture, genome stability, DNA repair, and quantitative imaging. I am particularly interested in understanding how the dynamic behaviour of chromosome-associated proteins is coupled to genome organization and function, and in extending these approaches toward mammalian chromosome biology and disease-associated mechanisms.

My experimental framework combines classical molecular biology with cutting-edge methodologies including:

Live-cell imaging and single-particle tracking
Chromatin immunoprecipitation coupled with sequencing (ChIP-seq)
Protein-protein interaction studies
Genetic manipulation of yeast strains
Quantitative analysis of chromosome dynamics

Employment

• Indian Institute of Technology Hyderabad PhD Scholar
  2021 - Present

Education

• Indian Institute of Technology Hyderabad PhD
  2021 - Present

Projects & Funding

Projects & funding information is unavailable.

Publications (4)

• The dynamic pool of Rec8-cohesin is crucial for meiotic recombination and transcription regulation in the yeast Saccharomyces cerevisiae
  Journal of Biological Chemistry 2026 DOI: 10.1016/j.jbc.2026.113064
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• Role of ATP-dependent chromatin remodelers in meiosis
  Trends in Genetics 2025 DOI: 10.1016/j.tig.2024.10.004
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• Single-molecule tracking dataset of histone H3 (Hht1) in Saccharomyces cerevisiae
  Data in Brief 2023 DOI: 10.1016/j.dib.2023.108925
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• In-vivo Single-Molecule Imaging in Yeast: Applications and Challenges
  Journal of Molecular Biology 2021 DOI: 10.1016/j.jmb.2021.167250
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