Rakesh Chatterjee

About

Hi
I am Rakesh Chatterjee and I come form West Bengal India. I did my Post graduation from the University Of Burdwan in 2008 in the field of Biotechnology. Then I completed my Ph. D in Biochemistry from CSIR Indian institute Of Chemical Biology in the year 2017 under Dr Saumen Datta. My primary area of research has been on structural studies of Type Three Secretion System (TTSS) associated proteins and their biophysical characterization using X-ray crystallography. In this context metabolic pathway that indirectly or directly influences this virulence signalling system has also been a matter of great interest to study . On this note we studied the allosteric features of Phosphopantethiene adenylyltransferase, one of the rate limiting enzyme of pantothenate pathway that has roles in regulating Type three secretion system in enterobactericeae. In my first postdoc in NISER, DAE India, I was part of Dr Rudresh Acharya Lab group and co investigated about how E.coli resist Copper mediated peroxide toxicity in phagocytosed macrophages during infection. We used biophysical and structural studies using X -ray diffraction to understand this biological mechanism. Presently at Umea University, under the supervision of Ignacio Mir Sanchis, I am involved in understanding how host proteins are involved in regulation and transfer of MGE (mobile genetic element) in MRSA ( Methicillin Resistant Staphylococcus Aureus) in the context of providing antibiotic resistance. For this I am using X-ray crystallography and CryoEM technique as a part of structural studies. My interest has been on proteins with Rossmann fold and studying them structurally in microbial system. A brief idea about my work experience is described as follows:

1. Biological mechanism of microbial system involved in virulence
2. Protein signalling pathways in prokaryotes and their cross-talks
3. Protein crystallization and X-ray diffraction studies
4. Molecular characterization of hypothetical genes using computational, biophysical and biochemical approaches
5. Modelling Enzymatic mechanism and assay development
6. Metal ion homeostasis.
7. Generation of soluble Membrane protein complexes and membrane biology.
8. Bio engineering
9. DNA Binding protein and Antibiotic resistance in MRSA.

Employment

• Umea Universitet Postdoctoral Scholar
  2019 - Present
• National Institute of Science Education and Research Postdoctoral Researcher
  2017 - 2019

Education

• University of Calcutta Ph.D Biochemistry
  2012 - 2017
• Indian Institute of Chemical Biology CSIR Ph.D Biochemistry
  2009 - 2017

Projects & Funding

Projects & funding information is unavailable.

Publications (6)

• Genome-wide survey and crystallographic analysis suggests a role for both horizontal gene transfer and duplication in pantothenate biosynthesis pathways.
  Biochimica et biophysica acta. General subjects 2019 DOI: 10.1016/j.bbagen.2019.05.017
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• Umbrella Sampling and X-ray Crystallographic Analysis Unveil an Arg-Asp Gate Facilitating Inhibitor Binding Inside Phosphopantetheine Adenylyltransferase Allosteric Cleft.
  The journal of physical chemistry. B 2018 DOI: 10.1021/acs.jpcb.7b09543
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• Transition of phosphopantetheine adenylyltransferase from catalytic to allosteric state is characterized by ternary complex formation in Pseudomonas aeruginosa.
  Biochimica et biophysica acta 2016 DOI: 10.1016/j.bbapap.2016.03.018
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• Identification and molecular characterization of YsaL (Ye3555): a novel negative regulator of YsaN ATPase in type three secretion system of enteropathogenic bacteria Yersinia enterocolitica.
  PloS one 2013 DOI: 10.1371/journal.pone.0075028
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• YspC: a unique translocator exhibits structural alteration in the complex form with chaperone SycB.
  The protein journal 2012 DOI: 10.1007/s10930-012-9426-5
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• Expression, Purification, Structural and Functional Analysis of SycB: A Type Three Secretion Chaperone From Yersinia enterocolitica
  The Protein Journal 2012 DOI: 10.1007/s10930-011-9377-2
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