Dr. Florence J Hardy

Employment

• University of Manchester Lecturer in Chemical Biology
  2026 - Present
• University of Washington Post Doctoral Research Scholar
  2024 - 2026
• University of Manchester
  2022 - 2024

Education

• University of Manchester PhD
  2018 - 2022
• University of Oxford MChem
  2012 - 2016
• The University of Manchester

Projects & Funding

Projects & funding information is unavailable.

Publications (24)

• Design of enantioselective photoenzymes by scaffolding mirror image catalytic sites
  2026 DOI: 10.64898/2026.10.16.759720
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• Atomic Functional Site Scaffolding in Biopolymers with RFdiffusion3
  Science 2026 DOI: 10.1126/science.aeh2982
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• From First Principles to Function: How AI Is Reshaping Enzyme Design
  Biochemistry 2026 DOI: 10.1021/acs.biochem.6c00356
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• Efficient and selective energy transfer photoenzymes powered by visible light
  Nature Chemistry 2025 DOI: 10.1038/s41557-025-01820-0
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• ­­­Photosensitizer Repositioning Affords an Enantiocomplementary Enzyme for [2+2]‐Cycloadditions
  Angewandte Chemie International Edition 2025 DOI: 10.1002/anie.202503576
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• Engineered enzymes for enantioselective nucleophilic aromatic substitutions
  Nature 2025 DOI: 10.1038/s41586-025-08611-0
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• Probing Ferryl Reactivity in a Nonheme Iron Oxygenase Using an Expanded Genetic Code
  Florence J. Hardy, Matthew G. Quesne, Emilie F. Gérard +10 more
  ACS Catalysis 2024 DOI: 10.1021/acscatal.4c02365
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• Noncanonical Amino Acids in Biocatalysis
  Chemical Reviews 2024 DOI: 10.1021/acs.chemrev.4c00120
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• A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme
  Nature Communications 2024 DOI: 10.1038/s41467-024-46123-z
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• Building Enzymes through Design and Evolution
  ACS Catalysis 2023 DOI: 10.1021/acscatal.3c02746
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• Mapping the Initial Stages of a Protective Pathway that Enhances Catalytic Turnover by a Lytic Polysaccharide Monooxygenase
  Jingming Zhao, Ying Zhuo, Daniel E. Díaz

  +18 more

  • Muralidharan Shanmugam
  • Abbey J. Telfer
  • Peter J. Lindley
  • Daniel Kracher
  • Takahiro Hayashi
  • +13 more
  American Chemical Society. Journal 2023 DOI: 10.1021/jacs.3c06607
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• Engineering Mutually Orthogonal PylRS/tRNA Pairs for Dual Encoding of Functional Histidine Analogues
  Protein science : a publication of the Protein Society 2023 DOI: 10.1002/pro.4640
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• A Non-Canonical Nucleophile Unlocks a New Mechanistic Pathway in a Designed Enzyme
  Research Square 2023 DOI: 10.21203/rs.3.rs-2922796/v1
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• A Designed Photoenzyme for Enantioselective [2+2]-Cycloadditions
  Nature. 2022 DOI: 10.1038/s41586-022-05335-3
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• Directed evolution of an efficient and thermostable PET depolymerase
  Nature Catalysis 2022 DOI: 10.1038/s41929-022-00821-3
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• A Designed Photoenzyme Promotes Enantioselective [2+2]-Cycloadditions via Triplet Energy Transfer
  Chemrxiv 2022 DOI: 10.26434/chemrxiv-2022-b4j5n
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• A Designed Photoenzyme Promotes Enantioselective [2+2]-Cycloadditions via Triplet Energy Transfer
  Research Square 2022 DOI: 10.21203/rs.3.rs-1650767/v1
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• Directed Evolution of an Efficient and Thermostable PET Depolymerase
  Research Square 2022 DOI: 10.21203/rs.3.rs-1350765/v1
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• The histidine-brace active site of a copper monooxygenase is redox active and forms part of an in-built enzyme repair mechanism
  Research Square 2022 DOI: 10.21203/rs.3.rs-1350705/v1
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• A Noncanonical Tryptophan Analogue Reveals an Active Site Hydrogen Bond Controlling Ferryl Reactivity in a Heme Peroxidase
  Mary Ortmayer, Florence J. Hardy, Matthew G. Quesne +8 more
  JACS Au 2021 DOI: 10.1021/jacsau.1c00145
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