MR

Mayank Roshan

National Dairy Research Institute,Karnal,Haryana,India.

Research areas: Medicine · Infectious Diseases · Milk Quality and Mastitis in Dairy Cows · Antimicrobial Resistance in Staphylococcus · Genetic and phenotypic traits in livestock

ORCID iD 0000-0002-6092-6783
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About

I am engaged in detecting Copy Number Variations of innate immunity genes across six cattle breeds using aCGH validated through qPCR. Proficient in microbiology research, focusing on antimicrobial resistance(AMR), biofilm characterisation, and phage therapy. Skilled in molecular biology techniques and experienced in Sanger sequencing data analysis and utilizing bioinformatics tools.

I have expertise in various fields of research pertaining to Microbiology (Antimicrobial resistance (AMR), Multidrug-resistant (MDR)bacteria isolation (MRSA, ESBL), Molecular identification (Drug-resistant genes, Enterotoxins), Antimicrobial susceptibility testing (AST), MIC determination, Spa typing, Biofilm characterization (MTP assay) and Mitigation of bacterial biofilm (Phage therapy and Phytochemicals from plants and their antibacterial activity), Molecular biology (DNA, RNA, Plasmid isolation, DNA purification, DNA quantification DNA Cloning and Agarose gel electrophoresis), Biophysical analysis (Polyacrylamide gel electrophoresis (PAGE), SDS-PAGE and Western Blot Analysis, Dot Blot, Antibody generation and purification, ELISA and Lateral flow assay), Genomics (Primer designing, Sanger sequencing data analysis and bioinformatics tools related to Genomics), Cell culture (Primary culture of Fibroblast cell and In-vitro fertilization (IVF) of buffalo, Cryopreservation and MTT assay). Presently I am working as a Research Associate (RA) under the NASF Project “Unique innate immunity genomic signatures identification in Sahiwal, Gir, Tharparkar, Kangeyam, Karan Fries and Holstein Friesian cattle using immunoformatics” at, ABC Division, NDRI Karnal, Haryana, India.
During the challenging COVID-19 pandemic, I designed the research work for two master's students. As a co-mentor, I contributed to the research strategy, designed and conducted experiments, analysed data, prepared and presented research findings, and contributed to the writing of a scientific manuscript. This work was published with me as an equal first author in Current Microbiology in April 2023 (PMID: 37120455). Additionally, I contributed to one of my master's students' research projects on the topic “Screening and Characterization of Phage for Mastitis-causing Multi-Drug Resistant Escherichia coli and Methicillin-resistant Staphylococcus aureus (MRSA),” which provided me with foundational knowledge in phage therapy. This experience equipped me with a comprehensive understanding of the challenges associated with bacteriophage research and honed my skills in sample collection, processing, and analysis. I have also worked on plant-based mitigation strategies to combat MDR, resulting in a publication in International Microbiology in March 2024 (PMID: 38568425).
I am excited about the opportunity to contribute to your esteemed research team. In addition, I have constantly displayed a strong work ethic as well as the ability to work efficiently both individually and as part of a varied team. I want the opportunity to continue my postdoctoral research on challenging projects in your laboratory. I believe that this employment will allow me to expand my research credentials and learn new scientific information to assist me in accomplishing my future ambitions.

Employment

  • National Dairy Research Institute,Karnal,Haryana,India. Research Associate
    2023 - 2024

Education

  • National Dairy Research Institute,Karnal,Haryana,India. PhD
    2017 - 2023

Projects & Funding

Projects & funding information is unavailable.

Publications (18)